Preparation of Tc-99m labeled vitamin C (Ascorbic acid) and biodistribution in rats

15th International Symposium on Radiopharmaceutical Chemistry -- AUG 10-14, 2003 -- Sydney, AUSTRALIAWOS: 000234790900001PubMed ID: 16394539The aim of this study was to label ascorbic acid with Te-99m and to investigate its radiopharmaceutical potential in rats. Ascorbic acid was labeled with Tc-99m using the stannous chloride method. The radiochemical purity of [Tc-99m]ascorbic acid (Tc-99m-AA) was determined by RTLC, paper electrophoresis, and RHPLC methods. The labeling yield was found to be 93 +/- 5.0%. The maximum labeling yield of Tc-99m-AA was determined at pH 5 and 25 degrees C. The biodistribution studies related to Tc-99m-AA were done in male albino Wistar rats. Tc-99m-AA, which has a specific activity of 13.02 GBq/mmol, was administered into the tail vein of the rats. The rats were sacrificed at 15, 30, 60, and 120 min after the injection by heart puncture under ether anaesthesia. The organs were weighed after removal. Their activities were counted using a Cd(Te) detector equipped with a RAD 501 count system. The %ID/g (% of injected dose per gram of tissue weight) in each organ and in blood was calculated. Maximum uptake of Tc-99m-AA was observed in prostate and kidneys at the 60th min. Tc-99m-AA may be a promising radio-pharmaceutical for the imaging of prostate and kidneys

Scientific Opinion on the update of the list of QPS‐recommended biological agents intentionally added to food or feed as notified to EFSA†

EFSA is requested to assess the safety of a broad range of biological agents in the context of notification formarket authorisation as sources of food and feed additives, food enzymes and plant protection products.The qualified presumption of safety (QPS) assessment was developed to provide a harmonised generic pre-assessment to support safety risk assessments performed by EFSA’sscientific Panels. The safety ofunambiguously defined biological agents (at the highest taxonomic unit appropriate for the purpose forwhich an application is intended), and the completeness of the body of knowledge are assessed. Identifiedsafety concerns for a taxonomic unit are, where possible and reasonable in number, reflected as‘qualifications’in connection with a recommendation for a QPS status. The list of QPS recommendedbiological agents was reviewed and updated in the current opinion and therefore becomes the valid list.The 2016 update reviews previously assessed microorganisms including bacteria, yeasts and viruses usedfor plant protection purposes following an Extensive Literature Search strategy. The taxonomic units relatedto the new notifications received since the 2013 QPS opinion, were periodically evaluated for a QPS statusand the results published as Statements of the BIOHAZ Panel.Carnobacterium divergens,Lactobacillusdiolivorans,Microbacterium imperiale,Pasteuria nishizawae,Pediococcus parvulus, Bacillusflexus,Bacillus smithii, Xanthomonas campestrisandCandida cylindraceawere recommended for the QPS list. Alltaxonomic units previously recommended for the 2013 QPS list had their status reconfirmed as well theirqualifications with the exception ofPasteuria nishizawaefor which the qualification was removed. Theexclusion offilamentous fungi and enterococci from the QPS evaluations was reconsidered but monitoringwill be maintained and the status will be re-evaluated in the next QPS Opinion update. Evaluation ofbacteriophages should remain as a case-by-case procedure and should not be considered for QPS statu